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The Drosophila Anion Exchanger (DAE) lacks a detectable interaction with the spectrin cytoskeleton
© Dubreuil et al; licensee BioMed Central Ltd. 2010
- Received: 15 March 2010
- Accepted: 23 June 2010
- Published: 23 June 2010
Current models suggest that the spectrin cytoskeleton stabilizes interacting ion transport proteins at the plasma membrane. The human erythrocyte anion exchanger (AE1) was the first membrane transport protein found to be associated with the spectrin cytoskeleton. Here we evaluated a conserved anion exchanger from Drosophila (DAE) as a marker for studies of the downstream effects of spectrin cytoskeleton mutations.
Sequence comparisons established that DAE belongs to the SLC4A1-3 subfamily of anion exchangers that includes human AE1. Striking sequence conservation was observed in the C-terminal membrane transport domain and parts of the N-terminal cytoplasmic domain, but not in the proposed ankyrin-binding site. Using an antibody raised against DAE and a recombinant transgene expressed in Drosophila S2 cells DAE was shown to be a 136 kd plasma membrane protein. A major site of expression was found in the stomach acid-secreting region of the larval midgut. DAE codistributed with an infolded subcompartment of the basal plasma membrane of interstitial cells. However, spectrin did not codistribute with DAE at this site or in anterior midgut cells that abundantly expressed both spectrin and DAE. Ubiquitous knockdown of DAE with dsRNA eliminated antibody staining and was lethal, indicating that DAE is an essential gene product in Drosophila.
Based on the lack of colocalization and the lack of sequence conservation at the ankyrin-binding site, it appears that the well-characterized interaction between AE1 and the spectrin cytoskeleton in erythrocytes is not conserved in Drosophila. The results establish a pattern in which most of the known interactions between the spectrin cytoskeleton and the plasma membrane in mammals do not appear to be conserved in Drosophila.
- Anion Exchanger
- Interstitial Cell
- Basal Plasma Membrane
- Larval Midgut
- Transposable Element Insertion
The spectrin cytoskeleton forms a submembrane protein scaffold that contributes to cell shape and membrane stability in the human erythrocyte [reviewed in ]. Biochemical studies identified the anion exchanger as the primary membrane anchor that attaches the spectrin cytoskeleton to the erythrocyte plasma membrane. Attachment is mediated by the protein ankyrin which serves as an adapter linking the N-terminal cytoplasmic domain of the anion exchanger to the β subunit of erythrocyte spectrin .
Subsequent studies of the spectrin cytoskeleton in more complex cells have uncovered a remarkable diversity of different membrane proteins attached to ankyrin. Many of these are physiologically important transporters and channels whose distribution in the cell is critical to function [3, 4]. Most of these integral membrane proteins appear to rely on their interaction with the spectrin cytoskeleton to be stably expressed at the cell surface. Consequently, mutations that knock out or inactivate ankyrin or spectrin lead to a dramatic reduction in their steady-state levels.
Spectrins and ankyrins are conserved between humans and Drosophila. There is a single conventional spectrin in Drosophila that is composed of α and β subunits arranged as a tetramer. Drosophila spectrin is nearly indistinguishable from human spectrin by electron microscopy, it possesses most of the known functional sites (e.g. actin-binding, ankyrin-binding, intersubunit interactions, PH domain, etc.) and it is found associated with the plasma membrane in most Drosophila cells that have been examined . Ankyrin is also conserved between Drosophila and humans. Ankyrins possess an N-terminal membrane binding domain composed of ankyrin repeats and a central spectrin binding domain. The two isoforms of ankyrin in Drosophila are similar to one another in their N-terminal and spectrin-binding domains, but their C-terminal domains are different, with further diversity added by alternative splicing of the neuronal ankyrin isoform DAnk2 [6–8]. Interestingly, there is comparable sequence diversity between mammalian ankyrin isoforms in the C-terminal domain with only limited similarity to Drosophila ankyrins [2, 7].
Yet, while spectrin and ankyrin are conserved in Drosophila, a remarkable divergence has become apparent in recent studies of candidate membrane anchors. Out of five interactions that have been examined so far only the interaction with L1 family cell adhesion molecules and ankyrin appears to be conserved in Drosophila. The L1 family member neuroglian possesses a conserved ankyrin-binding sequence in its cytoplasmic domain and it exhibits a functional interaction with ankyrin as well . Another cell adhesion molecule, E-cadherin, was recently shown to interact directly with ankyrin in mammals . In contrast, DE-cadherin (the fly counterpart of E-cadherin) does not appear to interact with ankyrin in Drosophila . Two other ankyrin-binding membrane proteins in mammals, voltage-dependent sodium channels and KCNQ potassium channels, are conserved in their transmembrane ion-conducting domains but the domains responsible for binding to ankyrin in humans are not conserved in Drosophila . The Na, K ATPase appears to be functionally linked to spectrin in Drosophila, since its behavior is altered in β spectrin mutants . However, while the connection appears to be mediated by ankyrin in mammals , ankyrin-binding activity does not appear to be required for the effect of spectrin on the Na, K ATPase in Drosophila .
To expand the repertoire of membrane proteins that can be analyzed, we took advantage of molecular tools generated by the Drosophila genome project. A homolog of the erythrocyte anion exchanger was identified in the genomic sequence of Drosophila. It was an attractive candidate for further analysis because of its well-known interaction with ankyrin in mammals. The anion exchanger belongs to a family of closely related genes (AE1, AE2 and AE3; also known as SLC4A1-3) and to a larger family of 10 related genes that transport bicarbonate (SLC4 A1-10 [ref. [16, 17]]). A conserved bicarbonate transporter (NDAE1) was previously identified in Drosophila and was shown to resemble the human sodium-dependent anion exchanger SLC4A8 . Here we describe the properties of a second Drosophila anion exchanger (DAE) that it is closely related to human SLC4A1-3. Based on sequence comparisons and protein localization experiments, it appears that the well-characterized interaction between AE1 and the spectrin cytoskeleton in human erythrocytes is not conserved in Drosophila.
Amino acid sequence analysis
Amino acid sequence comparison of human and Drosophila anion exchangers (% identity)
Sequence comparisons also revealed several notable features of the N-terminal and C-terminal cytoplasmic domains of DAE. Two regions of substantial sequence identity near the N-terminus (R and S, highlighted in red in Figure 2) were sandwiched between three domains of limited sequence identity. Some of this sequence conservation coincides with the cytoplasmic domain pH sensor of mammalian AE2 (region R [ref. ]). Sequence comparisons in this region established a hierarchy of sequence identities with the highest between human AE2 and AE3, the next highest between DAE and AE2/3, and the lowest between AE1 and the others. A 40 residue sequence within the first conserved region (R'; bold letters) has been noted previously for its conservation among anion exchangers [17, 23] and for its role in pH regulation of AE2 and AE3, but not AE1 . The R' sequence was nearly identical between AE2 and AE3, DAE was 80% identical to AE2 in this region, and AE1 was 53% identical to AE2. The other conserved region (S) exhibited the same overall pattern of sequence identities, further demonstrating divergence of AE1 relative to DAE.
Recent structural studies mapped an ankyrin interaction site within an 11 amino acid loop in the N-terminal cytoplasmic domain of AE1 (L in Figure 1; bold black characters in Figure 2 [ref. ]). That loop falls in between the zones of sequence identity described above. In fact, the sequence alignment between DAE and AE1 inserted a gap precisely at that site, because of the limited sequence homology between the two proteins in between regions A and B. Gaps were also introduced at this site in comparisons between human AE1, AE2 and AE3, suggesting that this binding site is not a conserved feature of the AE gene family.
The sequence LDADD near the C-terminus of AE1 (purple text) is thought to be responsible for a functional interaction between carbonic anhydrase and mammalian anion exchangers . A similar sequence was present in AE2 (LDANE), AE3 (LDSED), and in DAE (LDGSE), but not in NDAE1 (LDDIM). This pattern of sequence conservation further supports the grouping of DAE within the AE subfamily of anion exchangers.
Production and characterization of a DAE antibody
A polyclonal antiserum was produced in rabbits using a purified glutathione transferase fusion protein containing 140 amino acids from the cytoplasmic domain of DAE. The resulting antibody produced a robust response in western blots of the recombinant fusion protein (not shown). The antibody was affinity-purified before further use and cross-adsorbed with purified glutathione transferase.
The same antibodies were used for immunofluorescent staining of S2 cells expressing recombinant myc-tagged DAE. Staining of control cells with the DAE antibody produced very faint plasma membrane staining, close to the threshold of detection (not shown). However, both the myc tag and the DAE antibodies produced strong plasma membrane staining in transfected cells expressing recombinant DAE (Figure 3B). These results establish that the affinity purified DAE antibody detects the expected protein product and that DAE is a plasma membrane protein.
Localization of DAE in larval tissues
Closer inspection revealed that DAE staining in the copper cell region did not correspond to the copper cells themselves (Figure 4B). Instead, the copper cells were identifiable by their lack of staining with the DAE antibody, and by the bright staining of their apical and basolateral plasma membrane domains with the anti-α spectrin antibody (Figure 4C; merged in D). The DAE signal came from the spool-shaped interstitial cells found in between the copper cells in the middle midgut. Within the interstitial cells, DAE staining formed a gradient that was brightest at the basal surface of the cell and then diminished as it approached the perinuclear cytoplasm near the cell apex. This pattern corresponds to the elaborate infoldings of the basal plasma membrane that are seen by electron microscopy (not shown; ), which are most dense in the basal region region of the cell but in some cases extend nearly to the apical surface. A higher magnification view comparing the distribution of DAE (Figure 4F) to α spectrin (E) revealed that α spectrin staining was largely confined to sites of contact between copper cells and interstitial cells and to the banana-shaped apical invagination of the copper cell plasma membrane. The gradient of DAE staining in interstitial cells (Figure 4F) had no counterpart in the α spectrin staining pattern (E).
We identified and partially characterized a close relative of the vertebrate SLC4 anion exchangers in Drosophila and named it DAE. The amino acid sequence of this protein shares many of the characteristics of other members of this protein family, suggesting that it is likely to mediate Na-independent anion exchange in vivo. One major site of expression identified in this study is the stomach acid-secreting region of the larval midgut. We anticipate that there are other important sites of expression given that RNAi-mediated knockdown of DAE expression in the midgut was not lethal whereas ubiquitous knockdown of DAE with RNAi was lethal. Independent confirmation of the essential function of DAE comes from the recent identification of a recessive lethal transposable element insertion in the DAE gene (Flybase).
We previously speculated that a defect in anion exchange activity could account for the stomach acid secretion defect in Drosophila α spectrin mutants . This prediction was based on the known interaction of mammalian anion exchangers with ankyrin (and hence spectrin) and the known contribution of anion exchange to acid secretion in mammals. Targeted disruption of mouse AE2 clearly demonstrated an essential role in gastric acid secretion . Deletion of another anion exchanger gene family member (Slc26a9) also blocked gastric acid secretion in the mouse because of its likely effect on chloride secretion . Yet, a knockdown of DAE that was sufficient to eliminate detectable immunoreactivity had no detectable effect on the ability of larvae to produce stomach acid. Thus a different downstream target is likely to be responsible for the observed acid secretion defect in α spectrin mutants.
We set out to characterize DAE with the intent of using it as a membrane marker for the effects of spectrin mutations on interacting membrane proteins. Human erythrocyte AE1 is the best known membrane anchor for ankyrin and spectrin . We conclude from the present evidence that DAE is unlikely to interact with the spectrin cytoskeleton in Drosophila. Mammalian membrane proteins that interact with the spectrin cytoskeleton in vivo typically colocalize with spectrin and ankyrin by immunofluorescence. Using a sensitive antibody that readily detects spectrin in most Drosophila cells, we find that little or no spectrin is expressed in the interstitial cells where DAE is abundantly expressed. If spectrin is present and codistributes with DAE in interstitial cells it is below the threshold of detection of this antibody. Thus, we propose that a spectrin-independent mechanism is likely to explain the peculiar polarized distribution of DAE within basal invaginations of the interstitial cell plasma membrane. In mammalian MDCK and HBE cells, spectrin, ankyrin, the Na, K ATPase, and E-cadherin form a molecular complex and codistribute along lateral sites of cell-cell contact in these polarized epithelial cells [10, 35, 36]. However, in the anterior cells of the Drosophila midgut (AC), in which spectrin and DAE were both expressed, there was no detectable colocalization of the two proteins along lateral contacts between neighboring cells.
Amino acid sequence comparisons also failed to detect conservation of ankyrin-binding activity in DAE. The ankyrin binding site of human erythrocyte AE1 has been mapped to a loop within the N-terminal cytoplasmic domain . Yet, while there was remarkable amino sequence conservation among anion exchangers in regions flanking this loop. (regions R & S in Figure 2), the sequence of the ankyrin-binding loop itself was not conserved in DAE. The flanking sequence conservation is believed to reflect a pH sensing mechanism [24, 37], and is not thought to be related to ankyrin-binding activity. There is limited evidence suggesting an interaction between ankyrin and human AE2 and AE3 [38, 39]. But sequence comparisons failed to detect conservation of the ankyrin-binding site in these molecules either (not shown). Thus the ankyrin-binding sequence of AE1 may be a unique byproduct of erythrocyte evolution.
The apparent lack of an interaction between DAE and the spectrin cytoskeleton matches a pattern that has emerged in a number of recent studies. As described in the introduction, there are other membrane proteins with ankyrin-binding activity in mammals that do not appear to be conserved in Drosophila. We now add DAE to the list, leaving the L1 family cell adhesion molecule neuroglian as the only ankyrin-binding membrane protein whose interaction with ankyrin can be detected in Drosophila. What does this apparent lack of conservation mean? One possibility is that many of the known membrane interactions with the spectrin cytoskeleton arose through a physiological need that emerged in the course of vertebrate evolution. Thus, perhaps sodium channels and potassium channels do not require anchorage to the cytoskeleton to carry out their functions in Drosophila. Alternatively, it is possible that interactions between the spectrin cytoskeleton and integral membrane proteins are transient in evolution. Perhaps functional links can be swapped between different scaffold proteins such that membrane transporters in Drosophila are now linked to cytoskeletal scaffold proteins other than spectrin and ankyrin. Indeed, Drosophila spectrin function appears to be redundant in many of the cells that express it (manuscript in preparation), which may be conducive to rapid evolution of protein interactions. If so, it may turn out that different casts of membrane characters are associated with the spectrin cytoskeleton in Drosophila and mammals. Further insights into these issues are likely to emerge as the membrane anchors that attach the spectrin cytoskeleton to the plasma membrane in Drosophila are discovered and characterized.
Cloning and DNA sequencing
The full-length cDNA RE39419 was obtained from the Drosophila Genomics Resource Center. We extended the partial cDNA sequence data available in Flybase to establish that RE39419 belongs to mRNA class B (Figure 1). All sequence analysis was carried out in the DNA services facility at the University of Illinois at Chicago Research Resource Center. All oligonucleotide primers were obtained from Operon. DNA and protein sequence analysis was carried out using GCG software . Amino acid sequence alignments were created using the Gap program. The human anion exchanger accession numbers used were AE1 (X12609), AE2 (NM_003040), and AE3 (NM_005070).
A 420 bp BglII - EagI fragment from the cytoplasmic domain coding region was cloned in the pGEX-3X vector for expression in E coli DH5α. Expression studies in Drosophila S2 tissue culture cells were carried out using the pWUMB vector  which uses the Drosophila ubiquitin promoter to drive expression of an N-terminally myc-tagged product. A near-full-length BamHI fragment of the RE39419 cDNA clone was used. An internal BamHI site was inactivated by QuickChange mutagenesis to produce a silent mutation in the coding sequence. Both expression constructs were verified by DNA sequencing of the cloning junctions.
Production of DAE antibody
Expression of the glutathione transferase fusion protein with the cytoplasmic fragment of DAE was induced by IPTG and the protein was purified by affinity chromatography. Rabbits were immunized by popliteal lymph node injection of 50 ug purified antigen, followed by 50 ug antigen subdermally at one month intervals. Rabbits were pre-screened for lack of preimmune reactivity and they showed a marked response to the antigen by the second boost (not shown). Immune serum was affinity purified and antibody was counter-adsorbed with purified glutathione transferase for all of the studies described here.
Expression in S2 cells, western blots, and confocal microscopy
Transfection of S2 cells was carried out using lipofectamine (Invitrogen). Cells were processed for staining as previously described  using mouse monoclonal 9E10 (anti-myc; [ref. ]) and the affinity purified rabbit anti-DAE followed by Texas Red anti-mouse and FITC anti-rabbit secondary antibodies (Zymed Laboratories). Western blots were produced using standard methods, stained using the above antibodies with alkaline phosphatase-coupled secondary, and developed using BCIP and NBT (also from Zymed). Stained cells were viewed and photographed using an Olympus FV500 confocal microscope using a 60× plan-apo oil-immersion objective (N.A. 1.4) and Fluoview 2.1 software. Brightness settings were optimized for saturation using the photomultiplier and settings were kept constant in comparisons of transfected and untransfected cells.
We thank Srilakshmi Dhulipala for technical assistance. Supported by NIH GM49301 to RRD.
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