Figure 2

Amino acid sequence alignment between DAE and human erythrocyte AE1. The positions of 16 predicted transmembrane sequences are indicated in green boxes. The boundaries of the conserved cytoplasmic domain sequences R and S are indicated in red. The conserved subregion R' is indicated in bold red type. The sequence of the ankyrin-binding site in human AE1 is indicated in bold black type. Consensus glycosylation sites in the linker between transmembrane regions [5, 6] and [7, 8] are each marked by g. The site of interaction between the C-terminal domain of AE1 and carbonic anhydrase is indicated in purple.