Figure 3

Expression of endogenous and recombinant anion exchanger in S2 tissue culture cells. Western blots of total S2 cell proteins were stained with mouse anti-myc epitope antibody (lanes 1-3) or affinity pure rabbit anti-DAE antibody (lanes 4-5). Untransfected cells (0) were compared to transfected cells expressing myc-tagged recombinant β spectrin as a control (lane 2) or myc-tagged recombinant DAE (lanes 3 & 5). The predicted size of the anion exchanger was 136 kD. Transfected S2 cells expressing myc-tagged DAE were stained with the same antibodies and fluorescent secondary antibodies (bottom panels). Staining was primarily observed at the plasma membrane including filopodia.