Figure 1

Alternate protein products of the Drosophila anion exchanger gene. The six major classes of DAE protein products are depicted relative to the longest class (A). Classes K and J use an alternate splice site that removes part of the coding sequence near the N-terminus. Classes E and D use an alternate transcription start site and an internal methionine start codon. Classes B, J and D splice out an alternate exon which is located between two zones with high sequence identity (R and S). The short loop sequence (L) which is responsible for the interaction between AE1 and ankyrin in humans is not conserved in DAE. The boundaries of a short recombinant fragment of DAE expressed as a pGEX fusion protein are also shown.