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Fig. 7 | Journal of Negative Results in BioMedicine

Fig. 7

From: In vitro aggregating β-lactamase-polyQ chimeras do not induce toxic effects in an in vivo Caenorhabditis elegans model

Fig. 7

Expression levels of (a) BlaP-polyQ chimeras and (b) 82Q::YFP in transgenic strains expressing BlaP216Q55/79 and Q82::YFP in the body wall muscle cells. Twofold dilution series of pure BlaP197Q79 and YFP were analyzed in order to estimate transgenic expression levels using Western blot (mouse anti-polyQ antibody, 5TF1-1C2, Millipore). Additional bands in the (B) dilution series are observed, representing dimers of pure YFP. The Q82::YFP signal is observed at 38 kDa due to the additional weight of the polyQ region. Vertical lines were added to the image to aid in lane discrimination of the single immunoblot represented in the figure

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Journal of Negative Results in BioMedicine

ISSN: 1477-5751

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