Facial cutaneo-mucosal venous malformations can develop independently of mutation of TEK gene ﻿but may be associated with excessive expression of Src and p-Src

Brahami, Nabila; Subramaniam, Selvakumar; Al-Ddafari, Moudjahed Saleh; Elkaim, Cecile; Harmand, Pierre-Olivier; Sari, Badr-Eddine; Lefranc, Gérard; Aribi, Mourad

doi:10.1186/s12952-017-0072-5

Journal of Negative Results in BioMedicine

Table 2 Sequences of the sense and antisense primers used for direct sequencing of all the exons of the TEK gene

From: Facial cutaneo-mucosal venous malformations can develop independently of mutation of TEK gene but may be associated with excessive expression of Src and p-Src

Exon number	Sense and anti-sense primers 5’-3’
5’ UTR region	SP 5’-AGTCTGAGAAGGATTGGTCATCA-3’
5’ UTR region	ASP 5’-CTGTCTGAGCACAGGGAGTTT-3’
Exon 1.2	SP 5’-CAGCCCTGCTGATACCAAAT-3’
Exon 1.2	ASP 5’-CACTGATGAGATTTGGGGAGA-3’
Exon 2	SP 5’-GTTTACCCAATGGGGTCATG-3’
Exon 2	ASP 5’-AGCAGCTGCCAAGACAAAAG-3’
Exon 3	SP 5’-AACGCATTAGCCACCACTGT-3’
Exon 3	ASP 5’-ACATCTGCCCACAAGACCA-3’
Exon 4	SP 5’-CTGAATAGTTCAGCATTTTCATTCT-3’
Exon 4	ASP 5’-CAATGCCTGGTTTTTGCTTA-3’
Exon 5	SP 5’-CTCCTTGTCTTTGTTTCTGTCG-3’
Exon 5	ASP 5’-AAATTCTAGATCCAGCAACGATG-3’
Exon 6	SP 5’-GTTCATCCTACCATGCCACA-3’
Exon 6	ASP 5’-TGATTCAAAATCCTGTTGTCCA-3’
Exon 7	SP 5’-AGTTGGCATGATAGGAGCTCA-3’
Exon 7	ASP 5’-GGATGGAAACAAAAGAGGCTT
Exon 8	SP 5’-TCATCCACATCACAGGTGTCT-3’
Exon 8	ASP 5’-GTCAGTTCTGCCTCTCCAGG-3’
Exon 9	SP 5’-TGGGGTCAATGTTATGGACC-3’
Exon 9	ASP 5’-TCCTGGAAATTACCCCAAAG-3’
Exon 10	SP 5’-ATCACAAAACCTCAAAGCCG-3’
Exon 10	ASP 5’-AGCCACCACCTTGAGGTAGA-3’
Exon 11	SP 5’-TTTCAAAAGCCTAATTTTCCTCA-3’
Exon 11	ASP 5’-CACCCATTCAAAAGCGAACT-3’
Exon 12.1	SP 5’-AGTTGGCATGATAGGAGCTCA-3’
Exon 12.1	ASP 5’-GGATGGAAACAAAAGAGGCTT-3’
Exon 12.2	SP 5’-TGGGGTCAATGTTATGGACC-3’
Exon 12.2	ASP 5’-TCCTGGAAATTACCCCAAAG-3’
Exon 13	SP 5’-GCATAATGATCTAGGCCATGG-3’
Exon 13	ASP 5’-CCTATAGGGCTGCACGGTAA-3’
Exon 14	SP 5’-GCTGCTGTTAAGTTCCCATTACA-3’
Exon 14	ASP 5’-AAGCCAAAGAGAAGATGAGGC-3’
Exon 15	SP 5’-GTTCATCCTACCATGCCACA-3’
Exon 15	ASP 5’-TGATTCAAAATCCTGTTGTCCA-3’
Exon 16	SP 5’-TTTGGTTGTATACAGTTGATGGTGA-3’
Exon 16	ASP 5’-AGGCAAACCACAGCACAGTC-3’
Exon 17	SP 5’-GTTTACCCAATGGGGTCATG-3’
Exon 17	ASP 5’-AGCAGCTGCCAAGACAAAAG-3’
Exon 18	SP 5’-TCTTCTGCCAAGATGTGGTG-3’
Exon 18	ASP 5’-CAGGGGAGTACCTCGGAAA-3’
Exon 19	SP 5’-CTACCCAGCAATCATTTGTGG-3’
Exon 19	ASP 5’-TGCTAATTTATTTCCTGAGCTTTTT-3’
Exon 20	SP 5’-GTGCAAGGGCCTATCCTAGG-3’
Exon 20	ASP 5’-CCAAGTCACATCTGGTAGAACCA-3’
Exon 21	SP 5’-ATGTGCAGTGAGTTTGCCAA-3’
Exon 21	ASP 5’-CGGCTGACTTTGCTAGAGTC-3’
Exon22	SP 5’-GTTTACCCAATGGGGTCATG-3’
Exon22	ASP 5’-AGCAGCTGCCAAGACAAAAG-3’
Exon 23.1	SP 5’-AGGTGGAATCAAAGCAGCCT-3’
Exon 23.1	ASP 5’-CACGCCTTCCTATGAAGTCC-3’
Exon 23.2	SP 5’-AATCAGAATGCCTGTTTGTGG-3’
Exon 23.2	ASP 5’-TTCTTAGGCTTGTAAGCAATGAG-3’
3’ UTR region	SP 5’-TCTCAATTTTATCCCTCACCTG-3’
3’ UTR region	ASP 5’-TAAAGTATAATAAGGACATGTGGCA-3’

SP sense primer, ASP anti-sense primer, UTR untranslated region

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ISSN: 1477-5751

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