Fig. 4

a The effects of AAu1–AAu3 on excystation of A. castellanii belonging to T4 genotype. Briefly A. castellanii (5 × 10⁴ cyst) were incubated with AAu1–AAu3 in growth medium, PYG at 37 °C for 48 h. After this period, amoebae were counted using a haeamocytometer. Note that AAu1–AAu3 were unable to inhibit excystation. The dotted line represents the original inoculum. The results represent the mean ± standard error of two different experiments performed in duplicates. b Representative effects of AAu1–AAu3 on excystation of A. castellanii. The results are representative of three independent experiments. B1 is Amoeba alone; B2 is solvent alone (chloroform 15 μL); B3 is AAu1 (300 μM); B4 is AAu2 (300 μM); B5 is AAu3 300 (μM)