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Table 3 Operational features of the PCRs used for M. pneumoniae

From: Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria

Test (N) Sensitivity (%) Specificity (%) PPV (%) NPV (%)
mPCR NPA (77) N/A N/A N/A N/A
mPCR NPS (94) 23.1 (4.9–41.2) 97.1 (92.3–100) 75 (38.7–100) 76.7 (67.2–86.2)
mPCR IS (48) 57.1 (33.6–80.7) 77.8 (60.2–95.3) 66.7 (42.1–91.2) 70 (51.9–88.1)
Seeplex® PneumoBacter IS (48) 52.4 (28.6–76.1) 92.6 (80.7–100) 84.6 (61.2–100) 71.4 (55.0–87.8)
Speed-oligo® NPA/NPS (111) 11.1 (0–37.2) 96.1 (91.8–100) 20 (0–65.1) 92.4 (86.9–97.9)
  1. Values calculated with paired serologists as the gold standard. NPA Nasopharyngeal aspirate. NPS Nasopharyngeal swab. IS Induced sputum. PPV Positive predictive value. NPV Negative predictive value. N/A Not applicable (these values cannot be calculated for this test due to the lack of positive results in serology that coincide with the positive results in mPCR of NPAs)