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Table 2 List of Hsa21 Genes and peptides used to immunise rabbits.

From: Generation of a panel of antibodies against proteins encoded on human chromosome 21

Candidate Protein

Molecular weight of protein (kDa)

Peptide used to immunise rabbits

(protein accession numbers)

Mean antigenicity index

(Jameson and Wolf)

Double-stranded RNA-specific editase 1 (ADARB1)

80

Acetyl-CNHGSLQPRPPGLLSDPS-amide (ENSP00000374512, amino acid 478-495)

1.088889

B-1,3-galactosyltransferase 5 (B3GAL-T5)

36

A. Acetyl-K ERM VK GK QLK TFC-amide (ENSP00000343318, ENSP00000369994, ENSP00000369992, ENSP00000381699, ENSP00000403209 amino acid 82-93)

0.720833

  

B.Acetyl-C AA ETKEVDQ ESQR HG DI-amide (ENSP00000343318, ENSP00000369994, ENSP00000369992, ENSP00000381699, ENSP00000403209, amino acid 103-119)

1.547647

Protein Dopey 2 (DOPEY2)

258

Acetyl-CFRPVK QRYSVRNSV S-amide (ENSP00000382104, amino acid 455-470)

1.244375

Ribosomal RNA processing protein 1 homolog A (RRP1)

53

A. Acetyl-GDA LSQKRSE KP PAGSI C-amide (ENSP00000291569, amino acid 257-275)

1.745

  

B. Acetyl-C GARQRRRTPRPLTSARAKA-amide (ENSP00000291569, amino acid 428-446)

1.839474

Transient receptor potential cation channel subfamily M member 2 (TRPM2)

165, 177

Acetyl-C SW RLQ[Abu]P FGNND KQES L-amide (ENSP00000381023, ENSP00000300481, ENSP00000300482, ENSP00000393982, ENSP00000381026, amino acid 43-59)

1.105882

Superoxide dismutase 1 (SOD1)

16

Acetyl-FEQKE SNG PVKVW GSIC-amide (ENSP00000270142, amino acid 21-36, ENSP00000374645, amino acid 6-17)

0.9875

Ubiquitin carboxyl-terminal hydrolase 16 (USP16)

94

CST EEVDM KNI NMDN DLEV-amide (ENSP00000382857, ENSP00000382858, ENSP00000334808, amino acid 530-548)

0.930556

  1. Added conjugating cysteine are highlighted in bold, amino acids that are only found in human sequence are underlined. Abu, abbreviation for amino-n-butyric acid, used to substitute for an internal cysteine residue.