Figure 3
From: Generation of a panel of antibodies against proteins encoded on human chromosome 21
Affinity purified anti-RRP1 and SOD1 antibodies do not specifically label cells in the Tc1 mouse model. A similar pattern and intensity of staining is observed in adult Tc1 (A, C, E) and non-transchromosomic littermate control (B, D, F) cortical brain sections stained with affinity purified rabbit polyclonal antibodies generated against (A and B) RRP1 peptide B (9644-B), (C and D) SOD1 peptide (9638) and (E and F) SOD1 peptide (9637). Paraffin embedded sections were pretreated with protease prior to incubation with rabbit polyclonal antibodies, nuclei were counter-stained with haematoxylin. Scale bar = 50 μm.