Figure 2

Western blot of total brain proteins probed with affinity purified rabbit polyclonal antibodies. Total brain proteins from Tc1 adult mice (Tc1+), non-transchromosomic littermate control mice (C), transgenic mice that express wild type human SOD1 (Tg(SOD1)2Gur), transgenic mice that express mutant human SOD1 (Tg(SOD1*G93A)1Gur)and non-transgenic littermate control mice (control), recombinant human SOD1 wild-type (rSOD1) and human SOD1 G93A (rSOD1*G93A) mutant protein and human recombinant B3GALT5 (amino acids 29-128) conjugated to GST were used as indicated. To compensate for the high expression level of the human SOD1 transgene in the Tg(SOD1)2Gur and Tg(SOD1*G93A)1Gur mice a lower amount of total brain proteins were loaded per lane as indicated. Western blots were probed with affinity purified anti-bodies raised against (A) RRP1 peptide B (9644-B), (B) RRP1 peptide B (9643-B), (C) RRP1 peptide A (9644-A), (D) RRP1 peptide A (9643-B), (E) SOD1 peptide (9638), (F) SOD1 peptide (9637), (G) ADARB1 peptide (9528), (H) B3Gal-T5 peptide A (9598-A) and (I) B3Gal-T5 peptide B (9598-B). Closed arrows indicate band of interest, (A-I), open arrows indicate potential mouse SOD1 band (E and F) and 36 kDa band in PNGase treated samples (H).