Figure 1
From: Generation of a panel of antibodies against proteins encoded on human chromosome 21
Expression of candidate Hsa21 genes in the Tc1 adult brain. To determine if the candidate genes were expressed, RT-PCR was undertaken using primers against (B) USP16 (129 base pair product), (C) B3GAL-T5 (138 base pair product), (D) DOPEY2 (192 base pair product), (E) FTCD (207 base pair product), (F) ADARB1-primer set 1 (134 or 254 base pair product), (G) RRP1 (173 base pair product) (H) TRPM2 (181 base pair product) and (I) ADARB1- primer set 2 (156 base pair product) was undertaken. Primers against (A) human and mouse DYRK1A (235 base pair product) and (J) mouse Dyrk1a (793 base pair product) were used as controls. RNA from Tc1 adult mouse brains (Tc1), non-transchromosomic littermate control adult brains (C), human adult brain (HB) and human adult liver (HL) was used as indicated.