Figure 3From: Refractoriness of hepatitis C virus internal ribosome entry site to processing by Dicer in vivoHCV domains II, III and VI are processed into ~21 to 23-nt RNA species by recombinant human Dicer in vitro. (A) Predicted secondary structure of nt 1 to 515 of the HCV RNA genome. (B) Dicer RNase activity assays. 32P-labeled HCV RNA domain II (left panel), domain VI (center panel) or domain III (right panel) was incubated in the absence (-) or presence (+) of recombinant human Dicer (65 ng) with MgCl2. The samples were analyzed by denaturing PAGE and autoradiography. M, indicates a 10-nt RNA size marker.Back to article page