Table 1 Polymorphisms typed in the EDN, ECP, EPO and MBP genes.
From: Variation in genes encoding eosinophil granule proteins in atopic dermatitis patients from Germany
Gene | Rs number | Location | Amino acid | Primer | Annealing temperature | Restriction enzyme |
|---|---|---|---|---|---|---|
EDN | rs2013109 | Intron 1 | - | F: GGGTAAGTCAACGATCCCCAG R: GGTCTTGGTTATGACACACACTGTAGT | 69°C | HpyF10VI |
EDN | rs10132319 | Intron 1 | - | F: GGGTAAGTCAACGATCCCCAG R: GGTCTTGGTTATGACACACACTGTAGT | 65°C | AciI |
ECP | rs17792481 | Intron 1 | - | F: TGAGGGAGAGGTGAGCTGAAGT R: TGATGTGCTGGATGGCAAAC | 58°C | MboI |
ECP | rs2073342 | Exon 2 | T124R | F: TACGCTGCCCTCATAACAGAACT R: GAACTGGAACCACAGGATACCG | 58°C | PstI |
ECP | rs2233860 | 3' UTR | - | F: GTATGCAGACAGACCAGGAAGGA R: TTGGCAGATGAGTGATGATGAGTA | 61°C | RsaI |
EPO | rs11652709 | Exon 4 | Q122H | F: CTACCCTGGCCTGGAGTAGAAG R: CACGCACTTGTTGTTGCACC | 64°C | HpyF10VI |
EPO | rs3785496 | Intron 6 | - | F: ACTGGTTGTCACTTCCCCTCTC R: CAAAACCTTGCTTAAAACTCCCTT | 58°C | MseI |
MBP | rs490358 | Exon 2 | - | F: GGAAAAAACAGAGAAAGAAAGACTGAA R: CACTCCCACGGTCCCCTAAT | 58°C | BslI |
MBP | rs3741098 | Intron 3 | - | F: TGGTGGACAAAAACCTTACGTGTC R: ATGCTGGGGGCATATCCG A | 58°C | BsaHI |