Figure 5

Resinferotoxin (RTX) did not alter the pressure-discharge relations of regularly discharging, CAP-resistant BRs. On the left, the pressure-discharge relations for a representative, regularly discharging BR (COV = 1.9%, n = 677 points) that was exposed to RTX increasing concentrations (1, 10, 100, 1000, 10,000 nM) for 15 min each. The five pressure-discharge relations during RTX closely overlaid the original control relation (left panel). Instantaneous discharge values during 10,000 nM are colored yellow and all other datasets are black. The relationships completely overlapped. Average values for the discharge parameters (histograms on right) for seven regularly discharging BRs before and after 10,000 nM RTX are plotted. Pressure threshold (Pth), threshold frequency (Fth), slope sensitivity (Sth) and the maximum discharge frequency (Fmax) are displayed. Thus, unlike CAP, very high concentrations of RTX did not produce nonspecific effects on BR discharge.