Table 1 Description of PLIN SNPs, primers, PCR conditions and restriction enzymes.
From: Study of the impact of perilipin polymorphisms in a French population
SNP | Primers | PCR (bp) | MgCl2 (mM) | Ann. temp. | RE | Size (bp) for the wt allele | Size (bp) for the mut allele | MAF (%) |
|---|---|---|---|---|---|---|---|---|
rs4578621 | F : CAAGCTGGGTGCACTGGC R : GAGAAATAGAGGAATTAACC | 185 | 0.9 | 58 | Mnl I | 33+152 | 33+62+90 | 7.6 |
rs6496589 | F : CTGCCAACACTCG AGCTG R : ACCTGACTCTTCCTTGTCT | 110 | 1 | 60 | Pst I | 72+28 | no cut | 0.6 |
rs894160 | F1 : GCTGAGACTGAGTCACATGC R1 : GCTGAGACTGAGTCACATGC | 403 | 0.5 | 60 | - | - | - | - |
F2 : CTGTTTGTGGGGCTCCCTCG R2 : CCTCCCAGATCTTTTAAGAG | 126 (nested) | 0.5 | 52 | Xho I | 126 | 20+106 | 30.0 | |
rs8179070 | F : CAGCTATCTGCTGCCATC R : CTCACTGAACTTGTTCTCC | 219 | 0.5 | 58 | Msp I | 75+144 | 219 | ND |
rs3743373 | F : CAGCTATCTGCTGCCATC R : CTCACTGAACTTGTTCTCC | 219 | 0.5 | 58 | BseR I | 96+49+45+21+5+3 | 96+94+21+ 5+3 | ND |
rs8179071 | F : GTAGCAGCCCTGCCAGGCC R : CCGGCACGTAATGCACCAC | 248 | 0.5 | 69 | BstX I | 66+182 | 66+129+53 | 0.6 |
rs8179072 | F : GTAGCAGCCCTGCCAGGCC R : CCGGCACGTAATGCACCAC | 248 | 0.5 | 69 | Hpy188 III | 248 | 80+168 | ND |