On the genetic involvement of apoptosis-related genes in Crohn's disease as revealed by an extended association screen using 245 markers: no evidence for new predisposing factors

Table 3 Investigated SNPs in genes as represented by significantly differing microsatellites of the individual genotyping step.

Gene	rs#	Allele 01/02	Oligonucleotides (sense/antisense)	RE	TM (°C)	Allele: fragment length (bp)
FLIP	Rs7583529	A/C	GGTGATTATTCGGACCCCA/AACTACAGATCCCGTGTGGAG	TseI	57	01: 155 02: 103/52
	Rs2041765	T/C	GAACAAGGAGAGAACCTGGAC/GAGCTGGAAGGCACAGTACA	MboII	56	01: 309 02: 188/121
TNFRSF17	Rs3743591	A/G	ATAAGCAGTTTCTGTTTCAGATGT/CTCTACAAGAATTCCAGAGCA	BceAI	55	01: 223 02: 147/76
	Rs11570139	C/T	GCCCTGATATTTACACCCTGT/CAGCCATCTGCAACATGAT	CaiI	54	01: 269 02: 161/108
	Rs373496	T/C	AGGAACTGAAACTCACAATAGC/CAGCTCATTATCTGTCTGATGTT	AluI	55	01: 247 02: 100/90/54/3
MHC2TA	Rs3087456	G/A	* ¹ GTGAAATTAATTTCAGAGC TGT/CTCAGCTTCCCCAAGGAT	BfmI	58	01: 268 02: 231/37

Analyses were performed by using the RFLP method. The table depicts information on the used SNPs as well as RFLP/PCR conditions. * ¹ A 5'-tail was added to the mismatch (bold letter) sense primer (5'-CATCGCTGATTCGCACAT-3'). PCR was performed with a third oligonucleotide with the equal sequence as the tail. RE: restriction enzyme; TM: melting temperature (used for annealing in PCR).

ISSN: 1477-5751