Table 2 Transfection efficiency (% of live cells) in CD34-positive mononuclear cell cultures
From: Unsatisfactory gene transfer into bone-resorbing osteoclasts with liposomal transfection systems
1 h | 24 h | 48 h | 5 d | |
|---|---|---|---|---|
Baseline | 0 | 0,13 ± 0,031 | 0,47 ± 0,08 | 0 |
Adenovirus | 2,7 ± 0,12 | 13,1 ± 1,4 | 22,6 ± 3,4 | 4,2 ± 0,96 |
T1: Metafectene | 0 | 0,12 ± 0,042 | 0,23 ± 0,053 | 0 |
T2: Lipofectamine plus | 0 | 0,24 ± 0,060 | 0,30 ± 0,11 | 0 |
T3: Tfx-50 | 0 | 0,10 ± 0,037 | 0,41 ± 0,091 | 0 |
T4: FuGene6 | 0 | 0,23 ± 0,071 | 0,22 ± 0,13 | 0 |
T5: DOTAP | 0 | 0 | 0,56 ± 0,064 | 0 |
T6: DOSPER | 0 | 0,16 ± 0,046 | 0,28 ± 0,13 | 0 |
T7: JetPei | 0 | 0,20 ± 0,050 | 0,50 ± 0,15 | 0 |
T8: DuoFect | 0 | 0 | 0,12 ± 0,056 | 0 |