Figure 3

Number of new resorption pits in osteoclast cultures. Bone slices were biotinylated before cells were treated with transfection reagents for 2 h, followed by culture for 4 h, 8, or 24 h. Biotinylated resorption pits were visualized with FITC-labelled streptavidin and all resorption pits were stained with TRITC-WGA lectin. Resorption occurring after transfection was determined as pits emitting only red fluorescence. BL, baseline with no additions; Ad, adenoviral infection of GFP; T1-T8, transfection reagents as shown in Tables 1 and 2. The baseline control shown in the insert shows the staining pattern of the resorption pits before transfection (green) and overall resorption activity during the whole culture period (red). Yellow colour determines areas where both fluorochromes overlap. ANOVA: p < 0,001