Figure 2

Peroxidase immunostaining of cortactin in isolated rat Sertoli cells plated on Matrigel^® with the addition of 2 μM cytochalasin D and latex beads. Sertoli cell monocultures were incubated with 2 μM cytochalasin D and latex beads (arrows) for 2 hr (A) and 24 hr (B). At both time periods, the addition of cytochalasin D resulted in an apparent decrease in the amount of beads in Sertoli cell monocultures when compared to Sertoli cell monocultures incubated with beads but without cytochalasin D (inset). These positive controls were incubated for 2 hrs (inset in Fig 2A) and 24 hrs (inset in Fig 2B). Bar = 10 μm.