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Figure 3 | Journal of Negative Results in BioMedicine

Figure 3

From: Despite WT1 binding sites in the promoter region of human and mouse nucleoporin glycoprotein 210, WT1 does not influence expression of GP210

Figure 3

Determination of 5 prime end of GP210 mRNA. Transcription start site were determined analyzing nested PCR products generated with gene (fig. 5) and adapter specific primers. Lane 1, Marker. Lane 2, Nested PCR product obtained using two primer pairs specific to sequences within the adapter and the first exon. Lane 3, Negative water control. Lane 4, Positive control using bacterial adapter ligated cDNA and specific primers. Lane 5, Marker.

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