Figure 4

Elution Profiles of Hp Byproducts or Fractionated Pools from Anion Exchange Chromatography with Gradient Elution. Hp byproducts starting material (HpBP) or materials from fractionation via HIP peptide-1 (A) or ATIII (B) were applied to a 1-ml bed volume of DEAE sepharose in .05 M acetate, pH 4.0. Columns were washed with 10 column volumes of acetate buffer before application of LiCl gradients. All material bound to DEAE column and 100% eluted with LiCl. One-ml fractions were collected and analyzed for GAG and LiCl content as in "Materials and Methods." Arbitrary values of 100 were set for the elution peaks for each of the column runs with every other point multiplied by the same factor. The resulting plots are of LiCl concentration (X-axis) versus arbitrary amount of GAG (Y-axis).