Figure 3

Venus-FKBP12-Inp54p did not translocate to the membrane of DRG neurons after rapamycin treatment in knockin mice. A) CGRP-Inp54p^+/− (top) and Rosa-FRB^PLF/CGRP-Inp54p double heterozygous (bottom) mice were injected with rapamycin (i.t., three 1 nmol injections spaced evenly over 24 h, followed by paraformaldehyde perfusion shortly after the third injection). DRG were then dissected, sectioned, and mounted without immunostaining. B) Quantification of translocation. No significant difference in the membrane to cytoplasmic ratio of Venus-FKBP12-Inp54p in neurons containing FRB^PLF (n = 93 neurons) compared to neurons lacking FRB^PLF (n = 89 neurons). Scale bar, 20 μm.