In vitro studies of the influence of glutamatergic agonists on the Na+,K+-ATPase and K+-p-nitrophenylphosphatase activities in the hippocampus and frontal cortex of rats

Table 4 ATPases and p -NPPases activities in the hippocampus in the presence or absence of 300 μM of glutamatergic agonists

	K⁺- NPPase (10 mM)	Mg²⁺-pNPPase (10 mM)	Na⁺,K⁺-ATPase (9 mM)	Mg²⁺-ATPase (9 mM)	Na⁺,K⁺-ATPase (2 mM)	Mg²⁺-ATPase (2 mM)
Control	155.8 ± 7.4	52.6 ± 1.8	370.7 ± 26.2	205.4 ± 6.5	152.9 ± 13.9	149.7 ± 16.8
Glutamate	153.3 ± 6	56.2 ± 4.9	364.9 ± 32.9	189.4 ± 14.5	166.6 ± 21.5	136.3 ± 11.4
Control	175.0 ± 10.5	56.1 ± 2.4	348.7 ± 45.2	211.6 ± 16.8	156.1 ± 26.4	156.6 ± 13.6
NMDA	176.7 ± 13	57.0 ± 3.3	379.0 ± 21.7	195.8 ± 18.8	143.1 ± 18.5	155.6 ± 18.5
Control	129.7 ± 10.3	57.9 ± 5.8	360.8 ± 24.1	239.6 ± 13.2	146.1 ± 16.5	171.8 ± 23.5
AMPA	142.6 ± 16.3	55.8 ± 3.1	364.9 ± 19.7	230.0 ± 12.5	146.2 ± 12.1	165.5 ± 12.1
Control	189.0 ± 10.5	58.0 ± 3.3	345.6 ± 32	237.0 ± 16.3	180.1 ± 12.5	170.0 ± 22.6
Kainate	184.0 ± 9.8	60.8 ± 2.9	336.9 ± 44.7	221.2 ± 12.9	163.8 ± 22.6	164.3 ± 20.6

Values are expressed as mean ± standard deviation (5 animals/group). ATPases activities are expressed in nmol of P_i/mg protein/min, and p-PNPPases activities are expressed in nmol of p-nitrophenol/mg protein/min. The concentrations of the substrates are shown in parentheses.

ISSN: 1477-5751