In vitro studies of the influence of glutamatergic agonists on the Na+,K+-ATPase and K+-p-nitrophenylphosphatase activities in the hippocampus and frontal cortex of rats

Table 3 ATPases and p -NPPases activities in the frontal cortex in the presence or absence of 300 μM of glutamatergic agonists

	K⁺- NPPase (10 mM)	Mg²⁺-pNPPase (10 mM)	Na⁺,K⁺-ATPase (9 mM)	Mg²⁺-ATPase (9 mM)	Na⁺,K⁺-ATPase (2 mM)	Mg²⁺-ATPase (2 mM)
Control	162.2 ± 10	66.4 ± 3.3	303.8 ± 32.4	124.4 ± 13.2	154.0 ± 15.9	129.9 ± 16.1
Glutamate	177.5 ± 15	65.2 ± 5.8	343.6 ± 72.2	104.8 ± 17.2	149.2 ± 10.5	126.8 ± 10.7
Control	214.0 ± 23.2	65.1 ± 3.1	283.5 ± 30.4	192.8 ± 23.2	134.6 ± 15.9	139.3 ± 7.8
NMDA	215.5 ± 16.1	69.5 ± 6.7	270.6 ± 78.9	174.3 ± 31.7	130.7 ± 17.2	131.9 ± 12.5
Control	168.1 ± 13.6	61.6 ± 2.4	407.0 ± 53.4	219.7 ± 21.5	159.5 ± 10.9	128.1 ± 13.6
AMPA	170.1 ± 13.6	61.6 ± 5.4	383.7 ± 79.8	195.4 ± 36	146.3 ± 25	122.7 ± 10.1
Control	162.9 ± 6.9	66.2 ± 3.6	341.0 ± 39.8	192.0 ± 22.4	161.0 ± 17.9	129.0 ± 14.5
Kainate	171.3 ± 9.4	65.8 ± 7.6	346.1 ± 80.3	166.8 ± 16.3	148.8 ± 28.8	127.1 ± 10.5

Values are expressed as mean ± standard deviation (5 animals/group). ATPases activities are expressed in nmol of P_i/mg protein/min, and p-PNPPases activities are expressed in nmol of p-nitrophenol/mg protein/min. The concentrations of the substrates are shown in parentheses.

ISSN: 1477-5751