Table 2 Primer sequences, amplicon size and melting temperatures for the KCNE gene mutation screening
From: The KCNE genes in hypertrophic cardiomyopathy: a candidate gene study
Name | Sequence | amplicon size (bp) | Tm (°C) |
|---|---|---|---|
KCNE1.1F | 5' GCA GCA GTG GAA CCT TAA TG 3' | 225 | 58 |
KCNE1.1R | 5' CGG ATG TAG CTC AGC ATG AT 3' | Â | Â |
KCNE1.2F | 5' CTT CGG CTT CTT CAC CCT G 3' | 250 | 58 |
KCNE1.2R | 5' TTA GCC AGT GGT GGG GTT C 3' | Â | Â |
KCNE2.1F | 5' TCC GTT TTC CTA ACC TTG TTC 3' | 250 | 58 |
KCNE2.1R | 5' GCC ACG ATG ATG AAA GAG AAC 3' | Â | Â |
KCNE2.2F | 5' GAT GCT GAG AAC TTC TAC TAT G 3' | 300 | 58 |
KCNE2.2R | 5' GTC TGG ACG TCA GAT GTT AG 3' | Â | Â |
KCNE3F | 5' GCT AAG ATT TTA CCT GGG ATC TGA 3' | 626 | 65 |
KCNE3R | 5' TAT GCA CAA GGC TTC GGT CTA C 3' | Â | Â |
KCNE4F | 5' CTC TTG TCA GCT GTT TGG CGA ACC 3' | 886 | 65 |
KCNE4R | 5' CAC AGG CAC CTC CCG GAC TC 3' | Â | Â |
KCNE5F | 5' CCG CCG TGT CAC TCC CCG AAA 3' | 493 | 62 |
KCNE5R | 5' AGA TGA GGA GGG CGC GAA CCA 3' | Â | Â |