Figure 2

Example of false positive RT-PCR results. Example agarose gels of smeared amplicons from some shrimp samples. (a) Smeared amplicons (*) obtained from 2 shrimp samples tested for IMNV using the IQ2000 kit. The band at 680 bp is the kit internal control; -ve = negative control; M = DNA marker (2-log ladder, New England Biolabs). (b) Colony PCR screening of recombinant clones obtained from the bands similar to those marked by an asterisk (*) in (a). Underlined numbers represent clones subjected to sequence analysis. (c) Sequencing result for clone 10 in (b) with a 204 bp-insert. Portions of the sequence with homology to IMNV are underlined.