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Table 2 μCT analysis of 6-week-old male Ank +/+ and Ank KI/KI mice fed with normal and high Pi diet

From: Dietary phosphate supplement does not rescue skeletal phenotype in a mouse model for craniometaphyseal dysplasia

 

Normal Pi

High Pi

Parameters

Ank +/+(n = 4)

Ank KI/KI (n = 5)

Ank +/+(n = 7)

Ank KI/KI (n = 8)

Mandible

 Total volume (mm3)

0.28 ± 0.01

0.42 ± 0.02c

0.31 ± 0.01a

0.51 ± 0.04b, c

 Bone volume (mm3)

0.18 ± 0.02

0.31 ± 0.03c

0.23 ± 0.001a

0.44 ± 0.04b, c

 BVF (%)

66.5 ± 0.2

77.4 ± 2.2c

74 ± 2.3a

86 ± 0.01b, c

Femur trabecular bone (metaphyses)

 BVF (%)

5.9 ± 0.4

9.1 ± 0.4c

9.7 ± 3.1

9.9 ± 2.3

 Trabecular number (N/mm)

4 ± 0.01

5.3 ± 0.12c

5.27 ± 0.81a

5.66 ± 0.48

 Trabecular thickness (μm)

34 ± 2.4

37.6 ± 0.86c

36.2 ± 2.5

36.6 ± 2.2

 Trabecular Spacing (μm)

248.53 ± 19.49

186.9 ± 5.62c

193.19 ± 32.10a

173.35 ± 15.68

Femur cortical bone (diaphyses)

 Subperiosteal area (mm2)

1.64 ± 0.11

2.26 ± 0.22c

1.20 ± 0.32a

1.7 ± 0.10b, c

 Subendosteal area (mm2)

1.05 ± 0.06

1.46 ± 0.16c

0.88 ± 0.14

1.18 ± 0.09c

 Cortical porosity (%)

0.4 ± 0.2

1.95 ± 0.01c

0.4 ± 0.23

1.16 ± 0.01c

 Tissue density (mg/cm3 HA)

1135.0 ± 30.9

1125.0 ± 13.5c

1141.6 ± 11.1

1106.4 ± 16.1c

  1. BVF: bone volume fraction (bone volume/total volume)(%); a p < 0.05: statistical significance between Ank +/+ mice fed with normal and Pi diet;b p < 0.05: statistical significance between Ank KI/KI mice fed with normal and Pi diet;c p < 0.05: statistical significance between Ank +/+ and Ank KI/KI mice fed with the same diet. Data are mean ± SD