Fig. 3
From: Topical ocular dexamethasone decreases intraocular pressure and body weight in rats
a Gene expression of RGC markers in the rat retinas was unaltered after topical dexamethasone treatment. The relative mRNA level of RGC markers was compared in the eyes of rats treated with 0.1 % dexamethasone (DEX) or vehicle for 4 weeks (each N = 5). The expression of the RGC markers was normalized to Gapdh. Values represent the mean ± standard deviation. b The expression of ER stress markers in the rat anterior segment was unaltered after topical dexamethasone treatment. Topical dexamethasone did not elevate the ER stress markers CHOP and ATF4 in the rats. Protein levels of CHOP and ATF4 in anterior segment tissue (AST) were examined with an immunoblot analysis in the rat eyes treated with saline vehicle (Veh) or 0.1 % dexamethasone (DEX) for 4 weeks. Tunicamycin (Tunic)-treated-RGC5 cells were used as a positive control for ER stress induction. DMSO was used as a vehicle for the tunicamycin treatment in the RGC5 cells. Beta-actin served as the loading control (N = 3)