Figure 2

Chronic typical and atypical antipsychotics treatments effects in DARPP-32 levels. Western blot (A-B) and densitometric (C-D) analyses of DARPP-32 in PC12 WT and PC12 Clone cells treated HAL (1, 10 and 20 μM), CLO (10 μM) and RIS (20 μM) for 14 days [n = 5-7 per group]. Densitometric data of NCS-1 were normalized by Actin. The results show that there are no alterations in DARPP-32 protein expression levels followed by 14 days antipsychotic administration in PC12 WT [One Way ANOVA; P = 0.901] and PC12 Clone cells [One Way ANOVA; P = 0.919]. Data are means ± SD, *p < 0.05, One Way ANOVA. HAL, Haloperidol (1, 10 and 20 μM); CLO; Clozapine (10 μM); RIS, Risperidone (20 μM).