Genetic polymorphisms and susceptibility to lung disease

Lee, Pauline L; West, Carol; Crain, Karen; Wang, Lei

doi:10.1186/1477-5751-5-5

Journal of Negative Results in BioMedicine

Table 1 Primer List. List of primers used for DNA amplification and ASOH.

From: Genetic polymorphisms and susceptibility to lung disease

Primer name	Sequence	Temp °C
p47 Ex2F	GCTTCCTCCAGTGGGTAGTGGGATC	60
p47 161R	GGAACTCGTAGATCTCGGTGAAGC
Primer name	Sequence	Temp °C
p40 Ex2F	GTGCTGAGAGACGAATGTTGG	60
p40 Ex2R	GGGCAAGGTTCAGAGGTCAG
p40 Ex5F	GACGGGACATCTAGGCTGG	60
p40 Ex5R	GGCTCTGGCCATGTGGAAG
p40 Ex8F	TCTGAGGCGTGGCTCTGCTG	60
p40 Ex8R	GCTCATCTGGGAGCCACTGG
p40 Ex10F	ATGACACGGGCTTGTATCAGG	60
p40 Ex10R	GAGCTGAAGGTTTTTGCTGGTG
p40 86T	TGCTGACATCGAGGAGA	53
p40 86C	TGCTGACACCGAGGAGA	53
p40 353G	CCTGCTCAGCCTGCCGG	62
p40 353A	CCTGCTCAACCTGCCGG	61
p40 815C	ACGACCACCGCCCCTCA	58
p40 815T	ACGACCACTGCCCCTCA	56
p40 911C	GGACGTAGCGCTCATGG	57
p40 911A	GGACGTAGAGCTCATGG	55
Primer name	Sequence	Temp °C
p67 Ex3F	CTGGGCACCACAGGGAGCTA	58
p67 Ex3R	CACCAAGCCCGCAACACTGA
p67 Ex6F	GGGCTTCTATGTGGTTATCTCAA	60
p67 Ex6R	CCACAAGGAGGCTACCCTCTTCT
p67 Ex9F	GAGCCCAGGCAGGCTCAGTGTCAT	60
p67 Ex10R	GCCATCTCAAGGCGGGCTCAAGA
p67 Ex11F	GTGTTTCCCCACATCCAC	60
p67 Ex11R	AAGGCAGGGAGAGGAACT
p67 Ex13F	CAAGGGTTGGGCTAAAGGAC	60
p67 Ex14R	GTGTTCTCACACCACAGAGTCAG
p67 542G	TGTGGGCAGGCTGTTTC	55
p67 542A	TGTGGGCAAGCTGTTTC	53
p67 836C	CTGGGCCACGGTCATGT	57
p67 836T	CTGGGCCATGGTCATGT	55
p67 983G	CCCTGGAAGACCCCAGC	47
p67 983A	CCCTGGAAAACCCCAGC	47
p67 1105G	CTCAGCCCGGGCTCCCC	50
p67 1105A	CTCAGCCCAGGCTCCCC	50
p67 1167C	GCTGGAACACACTAAGCTG	54
p67 1167A	GCTGGAACAAACTAAGCTG	54
p67 1183C	CCAGCTATCGGCCTCGG	57
p67 1183T	CCAGCTATTGGCCTCGG	57
Primer name	Sequence	Temp °C
p22 Ex 2F	GACCCTGTCACTGTGCTGTG	61
p22 Ex 2R	GAGGCAAACAGCTCACTGTG
p22 Ex 3F	CTGAGCTGGGCTGTTCCTT	63
p22 Ex 3R	CCACCCAACCCTGTGAGC
p22 Ex 4F	CAGCAAAGGAGTCCCGAGT	60
p22 Ex 4R	GGAAAAACACTGAGGTAAGT
p22 Ex 5F	AAGGCTGAGAACACCCAGG	60
p22 Ex 5R	GCTCAGCCTACAGAGCCG
p22 Ex 6F	GACCCAGGTCCTGGCTGTG	60+DMSO
p22 Ex 6R	AGGCTCACGCGCTCCCGG
p22 85A	TCGTGGCCACAGCTGGG	59
p22 85G	TCGTGGCCGCAGCTGGG	59
p22 113T	GTGGTACTTTGGTGCCT	52
p22 113C	GTGGTACTCTGGTGCCT	52
p22 179A	GAAGAGGAAGAAGGGCT	51
p22 179C	GAAGAGGACGAAGGGCT	53
p22 214C	GACAGAAGCACATGACC	53
p22 214T	GACAGAAGTACATGACC	51
p22 403G	CGCCCATCGAGCCCAAG	59
p22 403A	CGCCCATCAAGCCCAAG	56
p22 521C	GCTGCGGCGGCGGCG	62
p22 521T	GCTGCGGTGGCGGCG	60
Primer name	Sequence	Temp °C
gp91phox Ex 9F	CTAAAGCAGAGATCTAAGTGG	61
gp91phox Ex 9R	ACGGTGACCACAGAAATAGCTACCT
gp91phox Ex 11F	GTTTCTAGGCATTCTGAGCATCAAG	61
gp91phox Ex 11R	GTTCGTAAGCCCTGTACACTATG
gp91phox Ex 12F	GTGCCTTGGTTAGAATAGCTTGTG	61
gp91phox Ex 12R	GTTGAAGATATCTGGAATCTTCTGTTG
gp91phox 907C	TGGTCACTCACCCTTTC	50
gp91phox 907A	TGGTCACTAACCCTTTC	48
gp91phox 1414G	ACAATGCCGGCTTCCTC	55
gp91phox 1414A	ACAATGCCAGCTTCCTC	53
gp91phox 1499A	GGAGAAAGATGTGATC	48
gp91phox 1499G	GGAGAAAGGTGTGATC	50
Primer name	Sequence	Temp °C
DUOX1 27F	AGAGAGATCTCCTCTCAAGG	58
DUOX1 27R	GGTCACCGGAAGAGCTGAG
DUOX1 28F	GGGACCTTGGAAGCTCCAG	58
DUOX1 28R	GGACGTCGAGAAGTGAAGAG
DUOX1 3532T	GGTCTGAGTTCCCCCAG	58
DUOX1 3532C	GGTCTGAGCTCCCCCAG	60
DUOX1 3647G	GCCGCCGCCGCAGTTTCC	66
DUOX1 3647A	GCCGCCGCCACAGTTTCC	63
Primer name	Sequence	Temp °C
DUOX2 Ex5F	ATGTTCTTTCCGACGTGGTGAG	63
DUOX2 Ex6R	GCGCCGCCCACATGAGCAG
DUOX2 Ex17F	GCCTGCTCAGACTCACAGAG	62
DUOX2 Ex17R	ACTCCTTAGGGATCTTGAGCAG
DUOX2 Ex24F	GATGCCTGCCAGATCCCCAG	62
DUOX2 Ex25R	TGGCCGCCGTGCCTCGTG
DUOX2 413T	TGGAGACCTCGTGTTCG	54
DUOX2 413C	TGGAGACCCCGTGTTCG	56
DUOX2 429A	CCGAACAGCGCGGGGAC	60
DUOX2 429C	CCGACCAGCGCGGGGAC	63
DUOX2 597-8GG	GCTTCTCGGGGGGACAG	58
DUOX2 597-8GA	GCTTCTCGAGGGGACAG	56
DUOX2 597-8CG	GCTTCTCCGGGGGACAG	58
DUOX2 597-8CA	GCTTCTCCAGGGGACAG	56
DUOX2 2048G	TGTGCTCCGTGTGGTCC	56
DUOX2 2048A	TGTGCTCCATGTGGTCC	54
DUOX2 3026G	CACTCCCCGGCTGTACA	56
DUOX2 3026A	CACTCCCCAGCTGTACA	52
DUOX2 3200T	CTTTGCCTTGCCACCCT	53
DUOX2 3200C	CTTTGCCTCGCCACCCT	55
Primer name	Sequence	Temp °C
TLR2 450F	ATTGCAAATCCTGAGAGTGG	58
TLR2 688R	GCAGTTCCAAACATTCCACG
TLR2 1141F	GCCTGTGAGGATGCCTGG	60
TLR2 1827R	GCACAGGACCCCCGTGAG
TLR2 1782F	GTGCTGTGCTCTGTTCCTG	60
TLR2 2392R	TCCCAACTAGACAAAGACTGG
TLR2 170T	GAAAAGATTTTGCTGGAC	53
TLR2 170Tdel	GAAAAGATTTGCTGGAC	53
TLR2 1892C	GGAAGCCCAGGAAAGCT	55
TLR2 1892A	GGAAGCACAGGAAAGCT	53
TLR2 2258G	CAAGCTGCGGAAGATAA	50
TLR2 2258A	CAAGCTGCAGAAGATAA	48
Primer name	Sequence	Temp °C
TLR9 Ex2F	GTGGGTGGAGGTAGAGCTG	60
TLR9 365R	ACAGCCAAGAAGGTGCTGG
TLR9 2501F	TGCTGCATCACCTCTGTGG	54
TLR9 2794R	TGCGGCTGCCATAGACCG
TLR9 13C	GTTTCTGCCGCAGCGCC	60
TLR9 13T	GTTTCTGCTGCAGCGCC	58
TLR9 237T	CACCTCCATGATTCTGA	52
TLR9 237G	CACCTCCAGGATTCTGA	54
TLR9 296C	GAACTGCCCGCCGGTTG	58
TLR9 296T	GAACTGCCTGCCGGTTG	60
TLR9 2588G	AAGTGGGCGAGATGAGG	57
TLR9 2588A	AAGTGGGCAAGATGAGG	55
TLR9 2644G	CGCAGAGCGCAGTGGCA	60
TLR9 2644A	CGCAGAGCACAGTGGCA	58
Primer name	Sequence	Temp °C
AAT Ex2F	TGTCGGCAAGTACTTGGCACAG	60
AAT Ex2R	CATAATGCATTGCCAAGGAGAG
AAT Ex3F	CAGATGATGAAGCTGAGCCTCG	65
AAT Ex3R	AGCCCTCTGGCCAGTCCTGATG
AAT Ex5F	GAGCAAGGCCTATGTGACAGG	60
AAT Ex5R	AGCTCAACCCTTCTTTAATGTCAT
AAT 374G	ACTCCTCCGTACCCTCA	56
AAT 374A	ACTCCTCCATACCCTCA	54
AAT 863A	GCACCTGGAAAATGAAC	50
AAT 863T	GCACCTGGTAAATGAAC	50
AAT 1096G	CCATCGACGAGAAAGGG	56
AAT 1096A	CCATCGACAAGAAAGGG	54

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ISSN: 1477-5751

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