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Table 3 Details of experiments on plasticity expressed in stimulus–response maps (Series II).

From: Searching for plasticity in dissociated cortical cultures on multi-electrode arrays

Protocol

Tetanus target

Probe amplitude

Conditions

No. and ages of cultures

Total expts.

Intervals

II.1

Single electrode.

Fixed, 0.8 V.

Baseline medium, spontaneous bursting.

4; 17–22 div

8

2 h

II.2

Single electrode.

Fixed, 0.8 V.

Bursts completely suppressed by 50 Hz background stimulation distributed over 20–40 electrodes, except during tetanization.

3a; 17–22 div

6

2 h

II.3

Single electrode.

Fixed, 0.8 V.

Spontaneous bursts suppressed by 1 mM magnesium.

3; 26–28 div

6

2 h

II.4

Single electrode.

Reduced (see Methods).

Spontaneous bursts suppressed by 2 mM magnesium.

4; 29–32 div

16

2 h

II.5a

8 electrodes, as in I.3a.

Range of voltages, 100–900 mV.

Spontaneous bursts suppressed by 1–2 mM magnesium.

3; 18–20 div

12

2 h

II.5b

8 electrodes, as in I.3b.

Range of voltages, 100–900 mV.

Spontaneous burst suppressed by 2 mM magnesium.

1; 17 div

4

2 h

  1. aIn a 4th experiment, burst suppression did not work sufficiently well. Those data were excluded from further analysis.