Figure 2From: Clock gene Bmal1 is dispensable for intrinsic properties of murine hematopoietic stem cells Cell cycling and differentiation of HSCs are normal in arrhythmic Bmal1 deficient mice. A, B) Normal frequency of HSCs in the BM of 8-10-week-old Bmal1 −/− mice. CD34−KSL fractions were assessed by flow cytometry. A) Data shown are representative of CD34−KSL cells at ZT5 and ZT17. B) The mean percentages ± SDs of CD34−KSL cells at ZT5 (n = 4) and ZT17 (n = 3) of two independent experiments. C) Comparable frequency of quiescent cells in HSC populations. HSCs of Bmal1 +/+ and Bmal1 −/− mice were stained with Pyronin Y and analyzed by flow cytometry to give the mean percentages ± SDs of Pyronin Y− cells in the CD34−KSL populations at ZT5 and ZT17 (n = 3) of two independent experiments. D) Normal EdU incorporation in Bmal1 −/− CD34−KSL cells. EdU was administered orally to mice for 3 weeks, and EdU incorporation into HSCs was evaluated using a Click-iT EdU PB Flow Cytometry Assay Kit. Data shown are the mean percentages ± SDs of EdU+ cells in HSC populations (Bmal1 −/− mice; n = 6, Bmal1 −/− mice; n = 3). E) White blood cell differentiation in young (10-week-old) and aged (40-week-old) mice. Each stack in the bar represents a cell type percentage. Gr-1+, granulocytes; Mac-1+, macrophages; B220+, B cells; CD4+, CD4+ T cells; and CD8+, CD8+ T cells (n = 6) of four independent experiments.Back to article page