In vitro studies of the influence of glutamatergic agonists on the Na+,K+-ATPase and K+-p-nitrophenylphosphatase activities in the hippocampus and frontal cortex of rats

Table 2 ATPases and p -NPPases activities in the hippocampus in the presence or absence of 50 μM of glutamatergic agonists

	K⁺- pNPPase (10 mM)	Mg²⁺-pNPPase (10 mM)	Na⁺,K⁺-ATPase (9 mM)	Mg²⁺-ATPase (9 mM)	Na⁺,K⁺-ATPase (2 mM)	Mg²⁺-ATPase (2 mM)
Control	164.3 ± 5.8	28.3 ± 6.5	339.1 ± 89.4	209.5 ± 34.6	198 ± 33.9	99.2 ± 7.4
Glutamate	166.9 ± 8.7	27.2 ± 5.4	355.3 ± 70.6	197.9 ± 36.4	202.8 ± 24.8	91.5 ± 8.9
Control	136.7 ± 13.4	40.8 ± 4.7	463.9 ± 67.1	159.3 ± 22.8	201.2 ± 32.6	94.5 ± 6.7
NMDA	140 ± 12.5	37.4 ± 7.1	432.5 ± 27.7	156.9 ± 38.5	190.4 ± 26.6	94.4 ± 6.5
Control	110.9 ± 12.7	41.5 ± 7.6	407.9 ± 61.9	238.8 ± 41.8	200.6 ± 26.6	79.7 ± 10.3
AMPA	114.4 ± 10.5	38.4 ± 5.6	412.1 ± 74	221.5 ± 42.9	188.3 ± 27.5	71.6 ± 6.3
Control	133 ± 5.2	43.7 ± 7.8	316 ± 119.4	144.9 ± 15.2	205.4 ± 21	83.6 ± 12
Kainate	131.9 ± 11.8	40.3 ± 8	374.7 ± 82.3	124.8 ± 19.7	205.5 ± 22.4	74.5 ± 7.8

Values are expressed as mean ± standard deviation (4–5 animals/group). ATPases activities are expressed in nmol of P_i/mg protein/min, and p-PNPPases activities are expressed in nmol of p-nitrophenol/mg protein/min. The concentrations of the substrates are shown in parentheses.

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