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Table 2 Enzymatic activity measurements

From: In vitro aggregating β-lactamase-polyQ chimeras do not induce toxic effects in an in vivo Caenorhabditis elegans model

Sample

Volume

Initial rate of

Hydrolysis (ΔA/min)

[BlaP197Q79] in the extract (nM)

Average [BlaP197Q79]

in the extract (nM)

Average concentration in 50 worms (~0.063 μL)

Replicate

 

1

2

3

1

2

3

  

BlaP197Q72 extract 1

330 μL

0.0056

0.0053

0.0056

5.71

5.46

5.73

5.63 ± 0.15

29.5 ± 0.8 μM

BlaP197Q72 extract 2

365 μL

0.0071

0.0062

0.0061

7.26

6.30

6.21

6.59 ± 0.58

38.2 ± 3.4 μM

  1. The initial hydrolysis rate of nitrocefin is given by ΔA.min−1, the slope of the graph representing the absorbance as a function of time when less than 10% of substrate is hydrolysed
  2. Based on the enzymatic activity measurements and assumptions detailed in the main text, an average concentration of 33.9 ± 6.2 μM BlaP197Q79 could be calculated