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Fig. 8 | Journal of Negative Results in BioMedicine

Fig. 8

From: In vitro aggregating β-lactamase-polyQ chimeras do not induce toxic effects in an in vivo Caenorhabditis elegans model

Fig. 8

Relative PolyQ protein levels as determined by dot blot analysis. Total polyQ protein levels of transgenic strains expressing BlaP197Q0, BlaP197Q58 and BlaP197Q72 in body wall muscle (BWM) cells (Punc-54), or ubiquitously expressed BlaP216Q55 and BlaP216Q79 (Prpl-28) were determined by dot blot analysis using an anti-polyQ antibody. Pure BlaP197Q79 or BlaP216Q79, as well as a transgenic strain expressing Q82::YFP in BWM were added as positive controls; protein extracts from wild-type worms function as a negative control. The signal was analysed using ImageJ. After background removal, the polyQ signal was normalized to the histone H3 signal. Each bar represents an individual biologically independent sample. The average of both wild type values was set to 1

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