Fig. 3

a) Nitrocefin assay of transgenic strains expressing A) BlaP197Q0/58/72 in the body wall muscle cells (BWM) and b) BlaP216Q0/55/79 in body wall muscle cells or ubiquitously supports functional BlaP expression. For each series, the first tubes represent the endogenous colour of nitrocefin before BlaP hydrolysis. Next, a negative control (extraction buffer), positive control (pure BlaP197Q79 and BlaP216Q79), extracts from wild-type worms and the transgenic BlaP strains are shown. Pictures were taken immediately after addition of the substrate, data are qualitative and show that all strains express functional β lactamase