Table 1 Oligonucleotide primers used for generation of DS-deficient decorin KI mouse
From: Absence of the dermatan sulfate chain of decorin does not affect mouse development
Purpose | Oligo name | Sequence (5’ > 3’) |
|---|---|---|
Generation of arms for mouse decorin homologous recombination | ||
|  | 5’arm_FWD | CACTTGAACTTCAGAGCCCAGGA |
|  | 5’arm_REV | GGCCAAGCACTCAAGAGAACTC |
|  | 3’arm_FWD | GGGCTGGACCATTTGAACAGA |
|  | 3’arm_REV | CCTTGGAGGTACGAATCATTGTG |
Mutagenesis of 5’ arm to introduce the S34A change (underlined base) | ||
|  | 5’arm_S34A_FWD | CTGGCATAATCCCTTATGACC |
|  | 5’arm_S34A_REV | CAGCCTCATCTTCTAGCATGAAG |
3’ genomic probe for Southern hybridization | ||
| Â | Probe_FWD | CCAAGGTATTTCCAACTCAGTG |
| Â | Probe_REV | CATGTGTGCCTAGTGATGATGG |
PCR genotyping and sequencing | ||
| Â | Genotyping_FWD | GGGCTGGACCATTTGAACAGA |
| Â | Genotyping_REV | GGGTGTGTCTCTTACTTCTGGA |