Fig. 1

Strategy for generation of decorin KI mutant mice. a A targeting fragment (10.6 kb) spanning exon 1, intron 1, exon 2 and part of intron 2 was inserted into the murine decorin gene by homologous recombination. The targeting construct contained a PGK-Neo selection cassette inserted at the BstZ171 site, and a single nucleotide mutation within exon 2 of the serine codon at the DS attachment site for an alanine codon (see c). b Southern blotting of EcoRV-generated fragments from genomic DNA of wild type (+/+), heterozygous (+/KI) and homozygous (KI/KI) mutant mice. The 3’ probe used lies outside the targeting construct. c Nucleotide sequencing of the decorin gene in the region bearing the S34A knockin mutation in heterozygous mice. A 263 bp PCR fragment was amplified with the indicated primer locations (semi-arrows) and sequenced. The reverse complement chromatogram matches the sequence shown at left