Fig. 4

NET-formation of PMA-stimulated primary neutrophils and 3 days DMSO-differentiated HL-60 cells. a NET formation by HL-60 cells differentiated with DMSO for 3 days and blood-derived neutrophils was assessed after stimulation with PMA for indicated periods of time. Results from 3 to 4 experiments are depicted as mean and SEM (n = 3–4). Comparison between nHL-60 cells and neutrophils at each time point was performed by unpaired, two-tailed t-test; #P < 0.05, ####P < 0.0001. b Representative images from experiments shown in (a). nHL-60 or blood-derived neutrophils were stimulated with PMA for 4 hours, fixed and stained with an antibody directed against histone-DNA-complexes and a secondary AlexaFluor488-labelled anti-mouse antibody (green). The nuclei were stained with DAPI (blue). The scale bar is 100 μm