Dietary phosphate supplement does not rescue skeletal phenotype in a mouse model for craniometaphyseal dysplasia

Table 2 μCT analysis of 6-week-old male Ank ^+/+ and Ank ^KI/KI mice fed with normal and high Pi diet

	Normal Pi		High Pi
Parameters	Ank ^+/+(n = 4)	Ank ^KI/KI (n = 5)	Ank ^+/+(n = 7)	Ank ^KI/KI (n = 8)
Mandible
Total volume (mm³)	0.28 ± 0.01	0.42 ± 0.02^c	0.31 ± 0.01^a	0.51 ± 0.04^{b, c}
Bone volume (mm³)	0.18 ± 0.02	0.31 ± 0.03^c	0.23 ± 0.001^a	0.44 ± 0.04^{b, c}
BVF (%)	66.5 ± 0.2	77.4 ± 2.2^c	74 ± 2.3^a	86 ± 0.01^{b, c}
Femur trabecular bone (metaphyses)
BVF (%)	5.9 ± 0.4	9.1 ± 0.4^c	9.7 ± 3.1	9.9 ± 2.3
Trabecular number (N/mm)	4 ± 0.01	5.3 ± 0.12^c	5.27 ± 0.81^a	5.66 ± 0.48
Trabecular thickness (μm)	34 ± 2.4	37.6 ± 0.86^c	36.2 ± 2.5	36.6 ± 2.2
Trabecular Spacing (μm)	248.53 ± 19.49	186.9 ± 5.62^c	193.19 ± 32.10^a	173.35 ± 15.68
Femur cortical bone (diaphyses)
Subperiosteal area (mm²)	1.64 ± 0.11	2.26 ± 0.22^c	1.20 ± 0.32^a	1.7 ± 0.10^b, ^c
Subendosteal area (mm²)	1.05 ± 0.06	1.46 ± 0.16^c	0.88 ± 0.14	1.18 ± 0.09^c
Cortical porosity (%)	0.4 ± 0.2	1.95 ± 0.01^c	0.4 ± 0.23	1.16 ± 0.01^c
Tissue density (mg/cm³ HA)	1135.0 ± 30.9	1125.0 ± 13.5^c	1141.6 ± 11.1	1106.4 ± 16.1^c

BVF: bone volume fraction (bone volume/total volume)(%); ^a p < 0.05: statistical significance between Ank ^+/+ mice fed with normal and Pi diet;^b p < 0.05: statistical significance between Ank ^KI/KI mice fed with normal and Pi diet;^c p < 0.05: statistical significance between Ank ^+/+ and Ank ^KI/KI mice fed with the same diet. Data are mean ± SD

ISSN: 1477-5751