Table 1 Amplification primers for qPCR
Gene | Forward primer | Reverse primer |
|---|---|---|
Fgfr1 | 5'- CCAGTGCATCCATGAACTCTGGGGTTCTCC-3' | 5'- GGTCACACGGTTGGGTTTGTCCTTATCCAG -3' |
Klotho | 5′- CTGGCTAAGGTTCAAGTACGGAGACCTCCC −3′ | 5'- GGAGCTGAGCGATCACTAAGTGAATACGCA -3' |
Npt2a | 5'- CTCATTGTGGGTGCCCAACATGATG -3' | 5'- ACCATGTGTCTCCCACGGACTGGAAG -3' |
Cyp24a1 | 5'- CTGCCCCATTGACAAAAGGC -3' | 5'- CTCACCGTCGGTCATCAGC -3' |
1αOHase | 5'- TCAGATGTTTGCCTTTGCCC -3' | 5'- TGGTTCCTCATCGCAGCTTC -3' |
18S | 5'-TTGACGGAAGGGCACCACCAG-3' | 5'-GCACCACCACCCACGGAATCG-3' |