Fig. 2

FGF23 in Ank ^KI/KI mice. a Immunohistochemistry showed no significant difference of FGF23 in femoral cortical bones from 16-week-old Ank ^+/+ and Ank ^KI/KI mice. Femurs stained with isotype IgG antibody and femurs from Fgf23 null mice were used as negative controls. FGF23-positive cells stained brown. Nuclei are counterstained with methyl green. Histogram shows numbers of FGF23-positive cells normalized to total number of osteocytes. Ank ^+/+ n = 5, Ank ^KI/KI n = 7; Scale bar = 100 μm. b ELISA assays of intact form of FGF23 in serum (top panel) and C-terminal form of FGF23 (bottom panel) in 3-, 10- and 16-week-old Ank ^+/+ and Ank ^KI/KI mice. 3-week-old mice: Ank ^+/+ n = 7, Ank ^KI/KI n = 5; 10-week-old Ank ^+/+ n = 10, Ank ^KI/KI =9; 16-week-old Ank ^+/+ n = 5, Ank ^KI/KI n = 5. c qPCR of Galnt3 expression in femoral bones of 16-week-old Ank ^+/+ and Ank ^KI/KI mice. Data are mean ± S.D. * p < 0.05