Fig. 1

Microparticle Flow Cytometry Quantification Method. a Side scatter profile of 200 nm silica size reference beads. b Annexin-V stained microparticles (MP) (red) and Annexin-V stained MPs treated with EDTA (black). The SSC threshold derived from the reference beads is represented by a dashed line (c-e) Representative flow cytometry data of cell specific antibodies to subtype and quantify endothelial, platelet, and macrophage MPs. We defined endothelial MPs as Annexin V+/CD31+ events, platelet MPs as Annexin V+/CD41+ events, and macrophage MPs as Annexin V+/CD45+ events. Red; specific antibody vs. black; isotype control. SSC; side scatter intensity