Fig. 3From: The rs361525 polymorphism does not increase production of tumor necrosis factor alpha by monocytes from alpha-1 antitrypsin deficient subjects with chronic obstructive pulmonary disease - a pilot studyTNF-α secretion by LPS-stimulated monocytes over three weeks. Monocytes from 3 healthy subjects (2 female) were isolated twice weekly for 3 weeks and stimulated in duplicate with 100 ng/ml of LPS for 3 hours. TNF-α concentration in the cell-free supernatant was measured using ELISA. Coefficient of variation (CV%) values for each subject are shownBack to article page