Calpain activity of Zucker skeletal muscle. Percentage of activated Calpain-3 (A) and µ-Calpain (B). Western blots show either μ-Calpain or Calpain-3 in obese and lean rats (C), with molecular mass markers, taken as a white light image prior to chemiluminescence and then images superimposed, indicated on the left. n = 6 for all groups. *indicates the non-specific band seen in rodent skeletal muscle with this antibody.