EBD staining in muscle fibres of Zucker rats. Proportion (%) of cross-sectional area that is EBD+ (A). Average Fluorescence intensity (arbitrary units) of EBD+ areas (B). Mean strength of EBD signal in myofibres and the interstitium as scored by a semi-quantitative Likert scale (C). Muscle sections of EDL (D), and Soleus (E) of obese and lean rats showing EBD accumulation around the skeletal muscle membranes of obese rats (solid arrows), with minimal penetration of the dye in lean rats. Magnification ×100. *p < 0.05 vs obese. #p < 0.05 vs EDL. n = 14 for each animal group.