Figure 5
From: Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes
Single-cell qPCR analysis of Mos knock-down and shRNA expression in mouse oocytes. (A) Relative Mos mRNA expression in oocytes from transgenic animals (Mos mRNA level in wild-type oocytes is set to 1). Rabbit β-globin mRNA, which was added to the lysis buffer at the time of collection, was used as an external standard for data normalization. Statistical significance of relative expression changes of Mos mRNA levels normalized to the β-globin was analyzed by the pair-wise fixed reallocation randomization test using the REST 2008 software. (B) Relative Mos_F shRNA expression in oocytes from transgenic animals. All data are expressed as mean +/- SEM from at least five oocytes.