Figure 4From: Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytesCharacterization of transgenic animals. (A) EGFP expression in brain, tail and kidney of transgenic animals. Bright-field images are shown to illustrate organ morphology. F1 generation mice from all transgenic lines were used for the analysis. EGFP expression in transgenic mice carrying a CMV-EGFP transgene (P.S., unpublished results) is shown for comparison. (B) EGFP expression in oocytes isolated form wild-type and transgenic animals. Bright-field images are shown to illustrate oocyte morphology. (C) EGFP expression in primary fibroblast isolated from wild-type and transgenic animals. NIH3T3 cells transfected with pZMP plasmid were used as positive controls.Back to article page