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Figure 3 | Journal of Negative Results in BioMedicine

Figure 3

From: Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes

Figure 3

Functional characterization of Mos-targeted shRNAs.(A) A schematic position of Mos-targeting shRNAs within the Mos mRNA. The Mos coding region is represented by an arrow. (B) HeLa cells were co-transfected with 50 ng of pLMP_LoxP plasmid expressing various Mos-targeting shRNAs and 50 ng of target Renilla luciferase plasmid carrying a fragment of Mos gene in sense orientation in the 3' UTR, and 50 ng of pGL4-SV40. Relative Renilla luciferase (RL) activity normalized to co-transfected untargeted firefly luciferase is shown. RL activity in the control sample (no shRNA-expressing plasmid) was set to 1. Values are expressed as mean +/- SEM from samples transfected at least in triplicates. Mos_F shRNA cloned into pSUPER plasmid is shown for comparison. (C) Same experimental design as in (B) except Renilla luciferase with antisense Mos target sequence in 3'-UTR was used as a reporter.

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